Synthesis and DNA binding studies of novel triazine-isatin hybrids: experimental and computational insights.

ABSTRACT: DNA binding is a crucial determinant in developing novel anticancer agents, as it plays a key role in the mechanism of action for many chemotherapeutic drugs. In this study, a series of novel s-triazine-isatin hybrids (7a-f) was synthesized, and their binding interactions with salmon sperm DNA (SS-DNA) were investigated under physiological conditions (pH 7.4) using UV-vis absorption spectroscopy. The experimental findings demonstrated strong DNA-binding affinity through absorption and intensity shifts via groove-binding modes with SS-DNA. The binding constants (K _b) of synthesized hybrids with SS-DNA calculated from the Benesi-Hildebrand plot, ranged from 10⁴ to 10⁵ M^-1, with compound 7f exhibiting the highest binding constan

SUBMITTER: Mushtaq A 

PROVIDER: S-EPMC11918252 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature