Time-Dependent Anabolic Response of hMSC-Derived Cartilage Grafts to Hydrostatic Pressure.

ABSTRACT: It is generally accepted that the application of hydrostatic pressure (HP) is beneficial for MSC chondrogenesis. There is, however, evidence to suggest that the timing of application might determine its impact on cell fate and tissue development. Furthermore, understanding how the maturity of engineered cartilage affects its response to the application of HP can provide critical insight into determining when such a graft is ready for in vivo implantation into a mechanically loaded environment. In this study, we systematically examined chondrogenic maturation of hMSCs over 35 days in the presence of TGF-β3 in vitro. At specific timepoints, the response of hMSCs to the application of HP following the removal of TGF-β3 was assessed; this partially models conditions

SUBMITTER: Chariyev-Prinz F 

PROVIDER: S-EPMC11919143 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature