Super-resolution microscopy based on the inherent fluctuations of dye molecules.

ABSTRACT: Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization microscopy and stimulated-emission-depletion microscopy often demand considerable resources. These methods depend heavily on elaborate sample-staining, complex optical systems, or prolonged acquisition periods, and their application in 3D and multicolor imaging presents significant experimental challenges. In the current work, we provide a complete demonstration of a widely accessible super-resolution imaging approach capable of 3D and multicolor imaging based on super-resolution optical fluctuation imaging (SOFI). We replace the confocal pinhole with an array

SUBMITTER: Krupinski-Ptaszek A 

PROVIDER: S-EPMC11919343 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature