Active remote focus stabilization in oblique plane microscopy.

ABSTRACT: Light-sheet fluorescence microscopy (LSFM) has demonstrated great potential in the life sciences owing to its efficient volumetric imaging capabilities. For long-term imaging, the light-sheet typically needs to be stabilized to the detection focal plane for the best imaging results. Current light-sheet stabilization methods rely on fluorescence emission from the sample, which may interrupt scientific imaging and add to sample photobleaching. Here, we show that for oblique plane microscopes (OPM), a subset of LSFM where a single primary objective is used for illumination and detection, light-sheet stabilization can be achieved without expending sample fluorescence. Our method achieves ∼21 nm axial precision and maintains the light-sheet well within the depth of focus of the detection system

SUBMITTER: Nguyen TD 

PROVIDER: S-EPMC11919345 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature