Exploiting the detector distance information in image scanning microscopy by phasor-based SPLIT-ISM.

ABSTRACT: Confocal microscopy is an important bio-imaging technique that increases the resolution using a spatial pinhole to block out-of-focus light. In theory, the maximum resolution and optical sectioning are obtained when the detection pinhole is fully closed, but this is prevented by the dramatic decrease in the signal reaching the detector. In image scanning microscopy (ISM) this limitation is overcome by the use of an array of point detectors rather than a single detector. This, combined with pixel reassignment, increases the resolution of 2 over widefield imaging, with relatively little modification to the existing hardware of a laser-scanning microscope. Separation of photons by lifetime tuning (SPLIT) is a super-resolution technique, based on the phasor analysis of the fluorescent signal i

SUBMITTER: Di Franco E 

PROVIDER: S-EPMC11919353 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature