Global emergence of Carbapenem-resistant Hypervirulent Klebsiella pneumoniae driven by an IncFII_K34 KPC-2 plasmid.

ABSTRACT:

Background

Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) has been increasingly reported worldwide, posing a severe challenge to public health; however, the mechanisms driving its emergence and global dissemination remain unclear.

Methods

We analysed CR-hvKp strains derived from canonical hvKp backgrounds, and acquired a carbapenemase-encoding gene. These strains were identified from 485 CRKp isolates in the CRACKLE-2 China cohort, 259 CRKp isolates from a multi-centre study, and 67,631 K. pneumoniae genomes available in GenBank. Clinical isolates harbouring the IncFII_K34 KPC-2 plasmid were selected for genome sequencing, RNA-Seq, conjugation assays, in vivo, ex vivo, and in vitro phenotypic characterisation.

Findings

Analysis of clinica

SUBMITTER: Jiang J 

PROVIDER: S-EPMC11919442 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature