Gasdermin E-mediated intestinal epithelial pyroptosis promotes chemically induced colitis in mice.

ABSTRACT:

Background

Gasdermin E (GSDME) is a newly identified pyroptosis executioner and is upregulated in the intestinal epithelial cell (IEC) of ulcerative colitis (UC) patients. However, the effects of epithelial GSDME on UC remain unknown.

Methods

Bone marrow chimera experiments were performed to investigate the role of GSDME in nonhematopoietic cells, mainly including IECs. An FITC-dextran assay was used to assess the integrity of the intestinal epithelial barrier.

Results

Gsdme^-/- chimeras that were reconstituted with wild-type bone marrow cells exhibited lower weight loss, disease activity index, colon shortening, and histology scores than wild-type chimeras after treatment with dextran sulfate sodium (DSS). However, Gsdme ^+/+ chimera

SUBMITTER: Wu YZ 

PROVIDER: S-EPMC11919448 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature