Three-dimensional matrix stiffness-based stem cell soil: Tri-phase biomechanical structure promoted human dental pulp stem cells to achieve pulpodentin regeneration.

ABSTRACT: The regeneration of the pulp-dentine complex is characterized by organizational diversity, with both dentine and pulp being essential for regenerating a complete tooth-like structure. Matrix stiffness plays a crucial role in guiding the multi-lineage differentiation of stem cells during the regeneration process. However, human dental pulp stem cell (HDPSCs) differentiation via three-dimensional (3D) matrix stiffness is still ambiguous. This study employed gelatin methacryloyl hydrogels of varying stiffness to investigate their effects on HDPSCs differentiation, and constructing a Tri-Phase Biomechanical Structure. The effects of 3D stiffness on HDPSCs proliferation, morphology, differentiation, and biomineralization were examined. The underlying mechanisms were analyzed by RNA sequencing (

SUBMITTER: Li X 

PROVIDER: S-EPMC11919457 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature