Identification of linear B-cell epitopes of Senecavirus A VP2 protein using monoclonal antibodies.

ABSTRACT:

Introduction

Senecavirus A (SVA) is an emerging vesicular pathogen in swine with clinical signs similar to those of foot-and-mouth disease, porcine vesicular disease and vesicular stomatitis, making the control of swine vesicular disease outbreaks difficult. Therefore, the development of effective diagnostics and vaccines for SVA has become critical. VP2 is a structural protein that elicits a strong immune response, which positions it a candidate for diagnostic and vaccine development.

Methods

In this study, five high-titer monoclonal antibodies (mAbs) were produced using hybridoma technology. Twenty-eight peptides covering the entire VP2 sequence were synthesised by overlapping peptide synthesis, and the positive peptides were screened with the five mAbs by ELISA and Dot-bl

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC11919882 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature