Establishment and application of an RNAi system in <i>Pichia pastoris</i>.

ABSTRACT:

Introduction

Reducing endogenous gene expression is key in microbial metabolic engineering. Traditional methods for gene knockout or suppression can be slow and complex. RNA interference (RNAi) provides a faster way to regulate gene expression using plasmids with hairpin RNA. This study examines single- and double-gene suppression in P. pastoris, a common system for expressing heterologous proteins. We also use reporter strains displaying EGFP on the cell surface to identify factors affecting protein secretion.

Methods

We established an RNAi system in P. pastoris by introducing plasmids containing hairpin RNA targeting specific genes. Reporter strains expressing EGFP on the cell surface were used to monitor the impact of gene suppression on protein secre

SUBMITTER: Ruan S 

PROVIDER: S-EPMC11919887 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature