Performance of electrochemical aptasensor as antigen test in clinical samples for early diagnosis of leptospirosis.

ABSTRACT: Early diagnosis of leptospirosis is critical for timely treatment and effective disease management. This study evaluated the diagnostic performance of a novel electrochemical aptasensor targeting the electron transfer flavoprotein subunit beta (EtfB) of Leptospira interrogans in clinical samples collected during the acute phase of leptospirosis. The aptasensor assay was tested using plasma samples and compared to the microscopic agglutination test (MAT), the standard reference method. To assess diagnostic performance, aptasensor results were evaluated against leptospirosis status as determined by MAT. Receiver operating characteristic (ROC) analysis identified a 40% decrease in electrochemical signal relative to the blank as the optimal cut-off, yielding an area under the curve (AUC) of 0.

SUBMITTER: Kositanont U 

PROVIDER: S-EPMC11920073 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature