HPV integration profiling using nanopore sequencing and association with cervical precancerous lesion.

ABSTRACT:

Objectives

HPV infection and HPV DNA integration can lead to cervical cancer, but the relationship with lesion severity is unclear. This study aimed to investigate the correlation between HPV integration profile and cervical lesion extent.

Materials and methods

Twenty patients representing cervicitis, CIN I, CIN II, and CIN III underwent nanopore sequencing for HPV genotype and integration site analysis. HPV integration profiles were correlated with lesion severity. Gene Ontology (GO) and KEGG analysis were used to identify stage-specific genes and pathways.

Results

HPV integration rates were 60, 60, 100, and 100% for cervicitis, CIN I, CIN II, and CIN III, respectively, with varying numbers of integrated genes. Each group had specific stage-related genes, with 83 sha

SUBMITTER: Hou Y 

PROVIDER: S-EPMC11920162 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature