Immunoelectrochemical assessment of human IgE in non-invasive samples of allergic individuals using PdNCs-labelled antibodies.

ABSTRACT: The escalating global prevalence of allergies presents a substantial public health challenge. Immunoglobulin E (IgE) serves as a key biomarker for allergic diseases, often measured in blood serum by ELISA immunoassays. Despite recent interest in minimally invasive sampling of biological fluids, the low sample volumes and IgE concentrations demand highly sensitive methodologies, typically confined to centralized laboratories. In this article, a decentralizable approach based on competitive immunoassays using Pd nanocluster (PdNCs)-labelled antibodies for electrochemical detection is proposed. With this aim, PdNCs were successfully bioconjugated with an anti-hIgE antibody to perform competitive immunoassays. To improve the analytical capabilities of the methodology, disposable screen-printed

SUBMITTER: Rodriguez-Penedo A 

PROVIDER: S-EPMC11920355 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature