RNA guanine content and G-quadruplex structure tune the phase behavior and material properties of biomolecular condensates.

ABSTRACT: RNA binding proteins (RBPs) are enriched in phase separated biomolecular assemblies across cell types. These RBPs often harbor arginine-glycine rich RGG motifs, which can drive phase separation, and can preferentially interact with RNA G-quadruplex (G4) structures, particularly in the neuron. Increasing evidence underscores the important role that RNA sequence and structure play in contributing to the form and function of protein condensates, however, less is known about the role of G4 RNAs and their interaction with RGG domains specifically. In this study we focused on the model protein, Fragile X mental retardation protein (FMRP), to investigate how G4-containing RNA sequences impact the phase behavior and material properties of condensates. FMRP is implicated in the development of Fragi

SUBMITTER: Vidal Ceballos A 

PROVIDER: S-EPMC11920403 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature