The Coordination Chemistry of Two Peptidic Models of NFeoB and Core CFeoB Regions of FeoB Protein: Complexes of Fe(II), Mn(II), and Zn(II).

ABSTRACT: Often necessary for efficient Fe(II) trafficking into bacterial cell, the Feo system is a vital transporter for many pathogenic bacteria and indispensable for proper development and survival in the host organism during infection. In this work, we present the metal-binding characteristics of the peptidic models of two putative Fe(II)-binding sites of E. coliFeoB: L1 (Ac-₄₇₇IMRGEATPFVMELPVYHVPH₄₉₆-CONH₂) being a fragment of the Core CFeoB region located between the transmembrane helices and L2 (Ac-₃₈VERKEG₄₃-CONH₂), which represents the ExxE motif found within the NFeoB domain. With a variety of physicochemical methods, such as potentiometry, mass spectrometry, NMR, and EPR spectroscopy, we have determined

SUBMITTER: Orzel B 

PROVIDER: S-EPMC11920956 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature