Assessing Modes of Toxic Action of Organic Cations in <i>In Vitro</i> Cell-Based Bioassays: the Critical Role of Partitioning to Cells and Medium Components.

ABSTRACT: High-throughput cell-based bioassays can fulfill the growing need to assess the hazards and modes of toxic action (MOA) of ionic liquids (ILs). Although nominal concentrations (C_nom) are typically used in an in vitro bioassay, freely dissolved concentrations (C_free) are considered a more accurate dose metric because they account for chemical partitioning processes and are informative about MOA. We determined the C_free of IL cations in AREc32 and AhR-CALUX assays using both mass balance model (MBM) prediction and experimental quantification. Partition coefficients between membrane lipid-water (K_mw), serum albumin-water (K_albumin/w), and cell-water (K_cell/w) as well as potential

SUBMITTER: Bae E 

PROVIDER: S-EPMC11921022 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature