Production of cinnamates and benzoates glucose esters by bioconversion using <i>Escherichia coli</i> expressing a glucosyltransferase from sweet potato.

ABSTRACT: Sweet potatoes (Ipomoea batatas) produce glucose esters from cinnamates during stress responses. We isolated a glucosyltransferase, IbGT1 (UGT84A20), from sweet potato and characterized its recombinant enzyme heterologously expressed in Escherichia coli. The recombinant IbGT1 enzyme reacted with cinnamates, especially p-coumaric acid, ferulic acid, and sinapic acid, and showed activity against benzoates with an optimum pH of 5.0-5.5. The enzyme also reacted with flavonoids under alkaline conditions (pH 8.0), with the main product being 7-O-glucosides. Bioconversion using E. coli expressing IbGT1 (Ec-IbGT1) as a whole cell biocatalyst converted 200 µM cinnamates such as p-coumaric acid and benzoates such as p-hydroxybenzoic acid into t

SUBMITTER: Kobayashi Y 

PROVIDER: S-EPMC11921131 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature