Heterologous production of corosolic acid, a phyto-insulin, in agroinfiltrated <i>Nicotiana benthamiana</i> leaves.

ABSTRACT: Triterpenoids, a group of specialized plant metabolites with substantial structural diversity, are promising for healthcare applications. Ursolic acid, a pentacyclic triterpenoid with therapeutic potential, is also important as a precursor of corosolic acid, which is known as a "phyto-insulin" for its insulin-like properties. Ursolic acid is synthesized from a linear 30-carbon precursor 2,3-oxidosqualene via cyclization to produce triterpene scaffold α-amyrin, followed by a series of oxidation steps at the C-28 position mediated by cytochrome P450 monooxygenases (CYPs) in the CYP716A subfamily. The Tsukuba system was developed for the high-level transient expression of foreign proteins in plant cells based on the use of a binary vector equipped with geminiviral replication system and a dou

SUBMITTER: Romsuk J 

PROVIDER: S-EPMC11921146 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature