From Hemp to CBD Crystals: A Scaled-Up Procedure for the Selective Extraction, Isolation, and Purification of Cannabidiol.

ABSTRACT: The isolation of cannabidiol (CBD) with a purity greater than 99%, while avoiding the presence of Δ9-tetrahydrocannabinol (THC), is essential for both exploring CBD applications and its commercialization. The scientific literature lacks robust and scalable protocols for obtaining pure CBD, instead primarily focusing on obtaining hemp extracts with higher cannabinoid content. Herein, we present a complete procedure for obtaining pure CBD crystals, starting from an industrial hemp cultivar. The protocol includes thermal decarboxylation of cannabinoids, supercritical CO₂ extraction, winterization of the extract, CBD purification via sequential C18 reverse-phase silica filtration, silica gel chromatography, and selective crystallization. Each step has been carefully optimized to ide

SUBMITTER: Calmanti R 

PROVIDER: S-EPMC11921148 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature