Sharp rise in high-virulence Bordetella pertussis with macrolides resistance in Northern China.

ABSTRACT:

Objective

To elucidate the evolution of antigen genotype and antimicrobial resistance distribution of Bordetella pertussis (B. pertussis) from 2019 to 2023 in northern China.

Methods

Polymerase chain reaction (PCR) amplification and sequencing were utilized to identify the seven antigen genotypes (ptxA, ptxC, ptxP, prn, fim2, fim3, tcfA). E-test and Kirby-Bauer (K-B) disc diffusion were employed to determine the minimum inhibitory concentration (MIC) and zone of inhibition for B. pertussis against antimicrobial agents. Subsequently, 50 isolates were chosen for multi-locus variable-number tandem-repeat analysis (MLVA) typing and whole-genome sequencing.

Results

A total of 442 B. pertussis isolates were determined. The strains with hig

SUBMITTER: Hu Y 

PROVIDER: S-EPMC11921162 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature