Microfluidic droplets with amended culture media cultivate a greater diversity of soil microorganisms.

ABSTRACT: Uncultivated but abundant soil microorganisms have untapped potential for producing broad ranges of natural products, as well as for bioremediation. However, cultivating soil microorganisms while maintaining a broad microorganism diversity to enable phenotyping and functional analysis of as diverse individual isolates as possible remains challenging. In this study, we developed and tested the ability of several culture media formulations that contain defined soil metabolites or soil extracts to maintain microorganism diversity during culture. We also assessed their performance in microfluidic droplet cultivation where single-soil microorganism isolates were encapsulated and cultivated in picoliter-volume water-in-oil emulsion droplets to enable clonal growth needed for downstream functiona

SUBMITTER: Dai J 

PROVIDER: S-EPMC11921321 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature