Gelling and reducing agents are potential carbon and energy sources in culturing of anaerobic microorganisms.

ABSTRACT: The majority of microorganisms in the environment have yet to be isolated in pure cultures, and the reasons behind this phenomenon remain elusive. In this study, we investigated the possibility of the commonly used gelling agent including agar and gellan gum as a source of carbon and energy in anaerobic roll-tube cultivation from one mangrove sediment sample and two high-temperature oilfield samples. Based on growth tests and genomic evidence, anaerobic gellan degraders were retrieved from genera of Clostridium, Lacrimispora, and lineages from the rarely cultivated phylum Atribacterota. Anaerobic agarolytic microorganisms were found to be members of Bacillus and Clostridium. We also proved the role of carbon and energy sources of L-cysteine, a routine agent used

SUBMITTER: Liu Y-F 

PROVIDER: S-EPMC11921371 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature