TK2268 encodes the major aminotransferase involved in the conversion from oxaloacetic acid to aspartic acid in <i>Thermococcus kodakarensis</i>.

ABSTRACT: Amino acid metabolism in archaea in many cases differs from those reported in bacteria and eukaryotes. The hyperthermophilic archaeon Thermococcus kodakarensis possesses an incomplete tricarboxylic cycle, and the biosynthesis pathway of aspartate is unknown. Here, four Class I aminotransferases in T. kodakarensis encoded by TK0186, TK0548, TK1094, and TK2268 were examined to identify the enzyme(s) responsible for the conversion of oxaloacetate to aspartate. Among the four proteins, the TK2268 protein (TK2268p) was the only protein to recognize oxaloacetate as the amino acceptor. With oxaloacetate, TK2268p only recognized glutamate as the amino donor. The protein also catalyzed the reverse reaction, the transamination between aspartate and 2-oxoglutarate. Substrate inhibition

SUBMITTER: Su Y 

PROVIDER: S-EPMC11921379 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature