Comparative assessment of a restored and natural wetland using ¹³C-DNA SIP reveals a higher potential for methane production in the restored wetland.

ABSTRACT: Wetlands are the largest natural source of methane (CH₄), a potent greenhouse gas produced by methanogens. Methanogenesis rates are controlled by environmental factors such as redox potential, temperature, and carbon and electron acceptor availability and are presumably dependent on the composition of the active methanogen community. We collected intact soil cores from a restored and natural freshwater depressional wetland on Maryland's Delmarva Peninsula (USA) to assess the effects of wetland restoration and redox shifts on microbial processes. Intact soil cores were incubated under either saturated (anoxic) or unsaturated (oxic) conditions and amended with ¹³C-acetate for quantitative stable isotope probing (qSIP) of the 16S rRNA gene. Restored wetland cores support

SUBMITTER: Hamovit N 

PROVIDER: S-EPMC11921397 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature