Establishment and characterization study of ovine mammary organoids.

ABSTRACT:

Background

The study of ovine lactation and mastitis is limited by the lack of physiologically relevant in vitro models. Compared to mammary epithelial cells, mammary gland organoids (MaOs) offer a better model for studying lactation, as they more accurately replicate the tissue structure and interactions of cells in vivo. Here, we optimize a protocol for the isolation, culture, characterization, and transfection of ovine MaOs derived from ewe mammary tissue.

Results

We compared different enzymatic digestion methods and found that type IV collagenase digestion for 60 min yielded the highest number of MaOs compared to a mixed enzyme solution of type II collagenase and hyaluronidase. Culture medium optimization revealed that medium B supported superior growth, with increased b

SUBMITTER: Feng R 

PROVIDER: S-EPMC11921478 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature