Extracellular vesicle-bound S100A8/A9 is differentially expressed in septic shock and prompts acute lung injury.

ABSTRACT:

Background

Sepsis is a common indirect insult leading to acute respiratory distress syndrome (ARDS). Circulating extracellular vesicles (EVs) have been reported to participate in the pathogenesis of sepsis. However, the alteration of EV-bound S100A8/A9 during septic shock, along with the role of S100A8/A9 in driving acute lung injury, remains unexplored.

Methods

EVs were isolated from the plasma of patients upon admission with sepsis or septic shock, as well as from healthy controls. Levels of EV S100A8/A9 were assayed via ELISA. To examine the effects and underlying mechanisms of septic shock EVs in acute lung injury, these EVs were administered intratracheally into wild-type C57BL/6 mice or mice with a deficiency of advanced glycation end-products (RAGE). In addition, a mo

SUBMITTER: Wang J 

PROVIDER: S-EPMC11921512 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature