RNA-binding protein SAMD4A targets FGF2 to regulate cardiomyocyte lineage specification from human embryonic stem cells.

ABSTRACT:

Background

RNA-binding proteins (RBPs) are essential in cardiac development. However, a large of them have not been characterized during the process.

Methods

We applied the human embryonic stem cells (hESCs) differentiated into cardiomyocytes model and constructed SAMD4A-knockdown/overexpression hESCs to investigate the role of SAMD4A in cardiomyocyte lineage specification.

Results

SAMD4A, an RBP, exhibits increased expression during early heart development. Suppression of SAMD4A inhibits the proliferation of hESCs, impedes cardiac mesoderm differentiation, and impairs the function of hESC-derived cardiomyocytes. Correspondingly, forced expression of SAMD4A enhances proliferation and promotes cardiomyogenesis. Mechanistically, SAMD4A specifically binds to FGF2 via a s

SUBMITTER: Yi N 

PROVIDER: S-EPMC11921648 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature