Enhancing myelinogenesis through LIN28A rescues impaired cognition in PWMI mice.

ABSTRACT:

Background

In premature newborn infants, preterm white matter injury (PWMI) causes motor and cognitive disabilities. Accumulating evidence suggests that PWMI may result from defected differentiation of oligodendrocyte precursor cells (OPCs) and impaired maturation of oligodendrocytes. However, the underlying mechanisms remain unclear.

Methods

Using RNAscope, we analyzed the expression level of RNA-binding protein LIN28A in individual OPCs. Knockout of one or both alleles of Lin28a in OPCs was achieved by administrating tamoxifen to NG2^CreER::Ai14::Lin28a^flox/+ or NG2^CreER::Ai14::Lin28a^flox/flox mice. Lentivirus expressing FLEX-Lin28a was used in NG2^CreER mice to overexpress LIN28A in OPCs. A series of behavioral tests we

SUBMITTER: Wu X 

PROVIDER: S-EPMC11921748 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature