Near-Infrared Probes Designed with Hemicyanine Fluorophores Featuring Rhodamine and 1,8-Naphthalic Derivatives for Viscosity and HSA Detection in Live Cells.

ABSTRACT: This paper presents the development of near-infrared (NIR) fluorescent probes, A and B, engineered from hemicyanine dyes with 1,8-naphthalic and rhodamine derivatives for optimized photophysical properties and precise mitochondrial targeting. Probes A and B exhibit absorption peaks at 737 nm and low fluorescence in phosphate-buffered saline (PBS) buffer. Notably, their fluorescence intensities, peaking at 684 (A) and 702 nm (B), increase significantly with viscosity, as demonstrated through glycerol-to-PBS ratio experiments. This increase is attributed to restricted rotational freedom in the fluorophore and its linkages to rhodamine or 1,8-naphthalic groups. Theoretical modeling suggests nonplanar configurations for both probes, with primary absorp

SUBMITTER: Olowolagba AM 

PROVIDER: S-EPMC11921759 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature