Application of a novel RNA-protein interaction assay to develop inhibitors blocking RNA-binding of the HuR protein.

ABSTRACT: RNA-protein interactions play an important regulatory role in several biological processes. For example, the RNA-binding protein HuR (human antigen R) binds to its target mRNAs and regulates their translation, stability, and subcellular localization. HuR is involved in the pathogenic processes of various diseases. Thus, small molecules blocking RNA-binding of HuR may be useful in a variety of diseases. Previously, we identified STK018404 as a small molecule targeting the HuR-RNA interaction. Based on this study we identified optimized compounds by exploiting combined structure-based and ligand-based computational approaches. To test a series of these compounds, we developed a novel readout system for the HuR-RNA interaction. Traditional methods to detect RNA-protein interaction come with s

SUBMITTER: Filcenkova L 

PROVIDER: S-EPMC11921777 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature