Development and use of DJ-1 affinity microcolumns to screen and study small drug candidates for Parkinson's disease.

ABSTRACT:

Background

DJ-1 is a protein whose mutation causes rare heritable forms of Parkinson's disease (PD) and is of interest as a target for treating PD and other disorders. This work used high performance affinity microcolumns to screen and examine the binding of small molecules to DJ-1, as could be used to develop new therapeutics or to study the role of DJ-1 in PD. Non-covalent entrapment was used to place microgram quantities of DJ-1 in an unmodified form within microcolumns, which were then used in multiple studies to analyze binding by model compounds and possible drug candidates to DJ-1.

Results

Several factors were examined in optimizing the entrapment method, including the addition of a reducing agent to maintain a reduced active site cysteine residue in DJ-1, the concent

SUBMITTER: Jones JC 

PROVIDER: S-EPMC11921869 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature