Vascular Microphysiological System for Investigating Endothelial Barrier Function During Organ Preservation and Reperfusion.

ABSTRACT: Endothelial cell damage after cold preservation and reperfusion injury causes deterioration of the endothelial barrier and ultimately results in edema, leading to transplant failure. Here, a vascular microphysiological system (MPS) is introduced as a testbed to investigate the combinational effect of thermal and fluid perturbations (i.e., wall shear stress) on human endothelial barrier function. Two methods of organ storage are compared: isochoric supercooling (ISC) preservation, which prevents ice formation at subzero temperatures; and, the standard clinical protocol of static cold storage (SCS) at 4 °C. Integrating electrical impedance measurements on chip allow real-time monitoring and quantification of barrier function during preservation and reperfusion protocols. Isochoric supercooli

SUBMITTER: Kim Y 

PROVIDER: S-EPMC11922018 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature