Cryo-Electron Microscopy Screening Automation across Multiple Grids using Smart Leginon.

ABSTRACT: Advancements in cryo-electron microscopy (cryoEM) techniques over the past decade have allowed structural biologists to routinely resolve macromolecular protein complexes to near-atomic resolution. The general workflow of the entire cryoEM pipeline involves iterating between sample preparation, cryoEM grid preparation, and sample/grid screening before moving on to high-resolution data collection. Iterating between sample/grid preparation and screening is typically a major bottleneck for researchers, as every iterative experiment must optimize for sample concentration, buffer conditions, grid material, grid hole size, ice thickness, and protein particle behavior in the ice, amongst other variables. Furthermore, once these variables are satisfactorily determined, grids prepared under identic

SUBMITTER: Sawh-Gopal A 

PROVIDER: S-EPMC11922482 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature