Effect of <i>AOX1</i> and <i>GAP</i> transcriptional terminators on transcript levels of both the heterologous and the <i>GAPDH</i> genes and the extracellular Y_p/x in <i>GAP</i> promoter-based <i>Komagataella phaffii</i> strains.

ABSTRACT: The constitutive and strong GAP promoter (P_GAP) from the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene has emerged as a suitable option for protein production in methanol-free Komagataella phaffii (syn. Pichia pastoris) expression systems. Nevertheless, the effect of the transcriptional terminator from the alcohol oxidase 1 gene (T_AOX1) or GAPDH gene (T_GAP) within the heterologous gene structure on the transcriptional activity in a P_GAP-based strain and the impact on the extracellular product/biomass yield (Y_p/x) has not yet been fully characterized. In this study, we engineered two K. phaffii strains, each harboring a single copy of a different combination of regulatory DNA elements (<

SUBMITTER: Viader-Salvado JM 

PROVIDER: S-EPMC11922483 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature