<i>Acanthamoeba castellanii</i>-Mediated Reduction of Interleukin-1β Secretion and Its Association With Macrophage Autophagy.

ABSTRACT: Noncanonical autophagy including unconventional protein secretion has been extensively studied. Our work focused on a leaderless IL-1β protein secretion from human macrophage in response to Acanthamoeba castellanii components, Acanthamoeba culture supernatant (CS) and cell lysate (CL), as well as its association with macrophage autophagy. Phorbol 12-myristate 13-acetate (PMA)-induced THP-1 macrophages were treated with Acanthamoeba components of pathogenic (ATCC50739) and nonpathogenic (ATCC30010) strains in vitro. The data showed that Acanthamoeba treatment resulted in low IL-1β secretion from macrophages. In addition, Acanthamoeba CL of both strains was able to upregulate autophagy-related (Atg) protein 8, an autophagy marker, whereas Acanthamoeba

SUBMITTER: Boonhok R 

PROVIDER: S-EPMC11922611 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature