Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.

ABSTRACT:

Introduction

Kawasaki disease (KD) is a medium-vessel vasculitis predominantly affecting children under 5 years of age and may involve the coronary arteries.

Methods

A mouse KD model was induced by Candida albicans cell wall extracts (CAWS), cardiac tissues were analyzed through integrated lactylomic and proteomic profiling. The lysine lactylation (Kla) results were normalized to the proteomic data.

Results

Elevated serum lactate and lactate dehydrogenase (LDH) levels were observed in KD patients. Given lactate's role as a substrate for Kla, this study investigated Kla modifications in KD. Proteomic analysis identified 150 upregulated proteins and 18 downregulated proteins, with 38.1% located in the cytoplasm and significant enrichment in immune-related pathway

SUBMITTER: Zhuo W 

PROVIDER: S-EPMC11922914 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature