Internalization of affinity tags enables the purification of secreted Chlamydomonas proteins.

ABSTRACT: There is great interest in establishing microalgae as new platforms for the sustainable production of high-value products such as recombinant proteins. Many human therapeutic proteins must be glycosylated, which requires their passage through the secretory pathway into the culture medium. While the low complexity of proteins in the culture medium should facilitate affinity purification of secreted recombinant proteins, this has proven challenging for proteins secreted by the unicellular green alga Chlamydomonas reinhardtii. In Leishmania tarentulae, we observed that C-terminally exposed affinity tags are frequently truncated, presumably due to proteolytic activity. We wondered whether this might also occur in Chlamydomonas and contribute to the difficulties in affinity purification of secr

SUBMITTER: Probst A 

PROVIDER: S-EPMC11923035 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature