ERG1A K⁺ channel increases intracellular calcium concentration through modulation of calsequestrin1 in C₂C₁₂ myotubes.

ABSTRACT: The ERG1A K⁺ channel modulates the protein degradation that contributes to skeletal muscle atrophy by increasing intracellular calcium concentration ([Ca²⁺]i) and enhancing calpain activity, but the mechanism by which the channel regulates the [Ca²⁺]i is not known. Here, we have investigated the effect of human ERG1A (HERG) on [Ca²⁺]i in C₂C₁₂ myotubes, using Fura-2 calcium assays, immunoblot, RT-qPCR, and electrophysiology. The data show that the rise in [Ca²⁺]_i induced by KCl-stimulated depolarization is of greater amplitude in C₂C₁₂ myotubes over-expressing HERG relative to controls, but this difference does not result from an increase in L-type channel (Ca_v1.1) Ca

SUBMITTER: Hockerman GH 

PROVIDER: S-EPMC11923081 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature