Orally delivered toxin-binding protein protects against diarrhoea in a murine cholera model.

ABSTRACT: The ongoing seventh cholera pandemic, which began in 1961, poses an escalating threat to public health. There is a need for new cholera control measures, particularly ones that can be produced at low cost, for the one billion people living in cholera-endemic regions. Orally delivered V_HHs, functioning as target-binding proteins, have been proposed as a potential approach to control gastrointestinal pathogens. Here, we describe the development of an orally deliverable bivalent V_HH construct that binds to the B-pentamer of cholera toxin, showing that it inhibits toxin activity in a murine challenge model. Infant mice given the bivalent V_HH prior to V. cholerae infection exhibit a significant reduction in cholera toxin-associated intestinal fluid secretion and

SUBMITTER: Petersson M 

PROVIDER: S-EPMC11923127 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature