Multiphasic condensates formed with mono-component of tetrapeptides via phase separation.

ABSTRACT: Biomolecular condensates, formed by liquid-liquid phase separation of biomacromolecules, play crucial roles in regulating physiological events in biological systems. While multiphasic condensates have been extensively studied, those derived from a single component of short peptides have not yet been reported. Here, we report the symmetrical core-shell structural biomolecular condensates formed with a programmable tetrapeptide library via phase separation. Our findings reveal that tryptophan is essential for core-shell structure formation due to its strongest homotypical π-π interaction, enabling us to modulate the structure of condensates from core-shell to homogeneous by altering the amino acid composition. Molecular dynamics simulation combined with cryogenic focused ion beam scanning el

SUBMITTER: Zhou L 

PROVIDER: S-EPMC11923152 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature