A telomere-to-telomere genome assembly of Chinese grain sorghum 654.

ABSTRACT: The grain sorghum inbred line 654 serves as a parent for numerous Chinese commercial hybrids and recombinant inbred lines (RILs), which have played a pivotal role in the cloning of several agronomically important traits. In this study, we present a telomere-to-telomere (T2T) genome assembly of the inbred line 654 (728.81 Mb) using PacBio HiFi, ultra-long Oxford Nanopore Technology, and Hi-C sequencing data. The T2T genome assembly has high integrity (contains all of 10 centromeres and 20 telomeres without any gaps), high contiguity (contig N90: 52.02 Mb), high completeness (98.33% BUSCO completeness, 98.88% k-mer completeness, and LAI 24.38), and extremely low base error (3.37 × 10^-7, QV: 64.72). A total of 62.34% sequences were identified as repetitive, and rest region containe

SUBMITTER: Wang F 

PROVIDER: S-EPMC11923156 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature