Ovarian cryopreservation with rapamycin improves fertility restoration in a murine orthotopic transplantation model.

ABSTRACT: Currently, the only fertility preservation option of prepubertal patients is ovarian tissue cryopreservation followed by autotransplantation (OTCTP). Once in remission and patients desire to conceive, autotransplantation of frozen/thawed tissue is performed. A major issue of this technique is follicular loss directly after transplantation, mainly due to follicle activation. Our previous research showed that adding rapamycin to the freezing medium counteracted follicle proliferation and activation induced by OTCTP in heterotopic autotransplantation of ovaries in mice. Our current study aimed to test the potential of this approach to improve fertility restoration in mice. Forty 4-week-old female C57BL/6 mice underwent unilateral oophorectomy followed by slow-freezing of ovaries with or witho

SUBMITTER: Bindels J 

PROVIDER: S-EPMC11923166 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature