3D bioprinted multi-layered cell constructs with gradient core-shell interface for tendon-to-bone tissue regeneration.

ABSTRACT: Rotator cuff tears are common among physically active individuals and often require surgical intervention owing to their limited self-healing capacity. This study proposes a new bioprinting approach using bone- and tendon tissue-specific bioinks derived from decellularized extracellular matrix, supplemented with hydroxyapatite and TGF-β/poly(vinyl alcohol) to fabricate engineered tendon-to-bone complex tissue. To achieve this goal, a core-shell nozzle system attached to a bioprinter enables the effective and simultaneous fabrication of aligned tendon tissue, a gradient tendon-bone interface (TBI), and a mechanically improved bone region, mimicking the native tendon-to-bone structure. In vitro evaluation demonstrated the well-directed differentiation of human adipose stem cells towar

SUBMITTER: Kim W 

PROVIDER: S-EPMC11923439 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature