A 96-Well Polyacrylamide Gel for Electrophoresis and Western Blotting.

ABSTRACT: Western blotting is a stalwart technique for analyzing specific proteins and their post-translational modifications. However, it remains challenging to accommodate more than ∼10 samples per experiment without a substantial departure from trusted, established protocols involving accessible instrumentation. Here, we describe a 96-sample Western blot that conforms to standard 96-well plate dimensional constraints and has little operational deviation from standard Western blotting. The main differences are that (i) submerged polyacrylamide gel electrophoresis is operated horizontally (similar to agarose gels) as opposed to vertically and (ii) a 6 mm thick gel is used, with 2 mm most relevant for membrane transfer (vs ∼1 mm typical). Results demonstrate that both wet and semidry transfer are co

SUBMITTER: Birtwistle MR 

PROVIDER: S-EPMC11923646 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature