Single Cell-Pair Proteomics for Decoding Immune-Cancer Cell Interactions.

ABSTRACT: The efficacy of cancer immunotherapy is significantly influenced by the heterogeneity of individual tumors and immune responses. To investigate this phenomenon, a microfluidic platform is constructed for profiling immune-cancer cell interactions at the single-cell proteomics level for the first time. Based on the platform, a comprehensive workflow is proposed for achieving accurate single-cell pairing of an immune cell and a cancer cell with low cell damage and high success rate up to 95%, cell pair co-culture, and real-time microscopic monitoring of the cell-pair interactions, cell pair retrieval, mass spectrometry-based proteomic analysis of singe cell pairs, and decoupling of the proteomic information for each cell within the cell pair with the stable-isotope labeling method. With the w

SUBMITTER: Xu QQ 

PROVIDER: S-EPMC11923901 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature