DNA methylation heterogeneity correlates with field cancerization and prognosis in lung adenocarcinoma patients.

ABSTRACT:

Background

Lung adenocarcinoma (LUAD) is the most common histological subtype of lung cancer. The distinctive genetic and epigenetic modifications in tumors and paired non-malignant samples, such as adjacent peri-tumor and tumor-distant normal lung tissues, have not been adequately studied.

Methods

We recruited 57 patients with resectable stage I-III LUAD and collected matched samples of the primary tumor, peri-tumoral tissues, and tumor-distant normal lung tissue. We performed bisulfite sequencing using a custom methylation panel to profile DNA methylation levels and obtained somatic variation landscape through targeted next-generation sequencing (NGS). We attempted to identify differential methylation blocks (DMBs) between the tumor, peri-tumor, and normal tissues.

Resu

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC11924610 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature