Enhancement of functional insulin-producing cell differentiation from embryonic stem cells through MST1-silencing.

ABSTRACT:

Background

Islet β-cell transplantation offers a promising treatment for repairing pancreatic damage in diabetes, with the transcription factor pancreatic duodenal homeobox-1 (PDX1) being crucial for β-cell function and insulin secretion. Mammalian threonine protein kinase (MST1) is recognized for its role in regulating PDX1 during cell apoptosis, yet its function in embryonic stem cell (ESC) differentiation into insulin-producing cells (IPCs) remain underexplored. This study investigated the effect of MST1-silencing on the differentiation of ESC into IPCs.

Methods

ESCs were transfected utilizing a recombinant MST1-silencing lentiviral vector (shMST1). qRT-PCR, immunofluorescence, flow cytometry, western blot and ELISA assays were performed to examine function of IPCs in vit

SUBMITTER: Song H 

PROVIDER: S-EPMC11924671 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature