Impact of phenytoin and valproic acid on cytotoxicity and inflammatory mediators in human mononuclear cells: with and without lipopolysaccharide stimulation.

ABSTRACT:

Background

Valproic acid (VPA) is known for its broad-spectrum antiepileptic effects and is recommended for generalized epilepsy, in contrast to phenytoin, which has a more limited spectrum. This study investigated the cytotoxic and inflammatory responses to phenytoin and VPA in peripheral blood mononuclear cells (PBMCs), with and without bacterial lipopolysaccharide (LPS) stimulation.

Methods

PBMCs from healthy donors were divided into 12 groups: control (Ctrl), phenytoin (Phy), and four concentrations of VPA (Val-50, Val-75, Val-100, Val-200), with and without LPS. Assessments were conducted on days 1 and 3, including total, live, and dead cell counts, cell viability, and lactic acid dehydrogenase (LDH) cytotoxicity assays. Inflammatory mediators (IL-6, IL-1β) and immune m

SUBMITTER: Alesawy A 

PROVIDER: S-EPMC11925041 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature