Deciphering key parameters enhancing lentiviral vector producer cells yields: Vector components copy number and expression.

ABSTRACT: The use of lentiviral vectors (LVs) in gene therapy is expanding, demanding high-quality viral preparations. Producer cell lines for LV production offer robust manufacturing platforms. However, their development is still progressing and more knowledge on the impact of vector components expression levels on vector yields and quality is essential. This work studies the impact of vector cassette expression and stability on vector titer and quality, identifying key parameters in cell line development. Ten heterogeneous LV stable producer clones established through random cassette integration were characterized. The gag-pol and rev cassettes, expressed under the control of constitutive promoters, showed robust expression generating titers of 10⁹ physical particles (P.P.

SUBMITTER: Formas-Oliveira AS 

PROVIDER: S-EPMC11925171 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature