The Secreted Ly6/uPAR-Related Protein-1 (SLURP1) Protects the Cornea From Oxidative Stress.

ABSTRACT:

Purpose

Previously, we reported that the secreted Ly6/uPAR-related protein-1 (SLURP1), abundantly expressed by the corneal epithelium (CE) and secreted into the tear fluid, suppresses NF-κB signaling in healthy corneas and is downregulated in response to a variety of stressors, allowing helpful inflammation to progress. Here we investigate whether SLURP1 manifests its broad protective effects by promoting corneal redox homeostasis.

Methods

Oxidative stress was induced in the wild-type (WT) and Slurp1-null (Slurp1X-/-) mouse corneas using 1350 J/m2 UV-B, and in human corneal limbal epithelial (HCLE) and SLURP1-overexpressing HCLE-SLURP1 cells with 100 J/m2 UV-B, 0.4 µg/mL mitomycin-C, or 0-100 µM H2O2. We evaluated their (i) redox status (GSH:GSSG ratio) using O-phthalaldehyd

SUBMITTER: Kaur S 

PROVIDER: S-EPMC11925223 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature